Black Garlic fights Cancer

CreekBay Black Garlic
www.black-garlic.net
Black Garlic PropertiesBlack garlic contains allicin, a powerful sterilization function, and inhibition of platelets in the blood to prevent the function of generating blood clots. Allicin and vitamin B1, a combination of garlic, thiamine, has the role of fatigue, to promote the decomposition of carbohydrate, inhibition of blood glucose levels. It also contains a substance called gourd garlic sulfur compounds, which can activate cells, auxiliary vitamin B1 carbohydrate conversion into energy, fatigue, build up their strength. Black Garlic is also rich in the mineral selenium, and elimination of reactive oxygen species that cause various diseases of affluence, the antioxidant can prevent cancer.

Black garlic has ten times the benefits of regular garlic and so many other properties. It has the ability to help your body fight CANCER, to lower blood pressure and improve blood flow. This is just a short list of its benefits. Millions of Americans are taking garlic pills because of they believe in the health benefits. Now there is a product that is 10 TIMES stronger!

The taste is fruity and does not taste like garlic at all. It has the consistency of a dried fruit, so it is like eating raisins. The fermented garlic can be used in or on anything from ice cream, pizza to any savory meal. We have made ice cream with it and the kids loved it, our curry paste is a hit every time we serve it. It has been used on shows like Top Chef and Chopped.

You can get black garlic in its natural state (garlic bulb that you need to peel), already peeled, in paste, in powder, in pill form and as a liquid. This very versatile little gem is not only good for you but can be used in any food preparation.

For all the health benefits it is best to take in pill form to ensure that you get enough each day.

18 Essential Amino Acids – (Isoleucine, Leucine, Lysine, Methionine, Cyctine, Phenylalanine, Tyrosine, L-hydroxybutyrate, Tryptophan, Serine, Proline, Glycine, Glutamine, Aspargine, Alanine, Histidine and Valine).

Table contents of amino acids in garlic and black garlic

Vitamins B-1, B-2 B-6, C and E
Supper Oxide Dismutase, (SOD)
S-Alycisteine (SAC)
Prostaglandins E1 (This series of prostaglandins relax blood vessels, improve circulation, lowers blood pressure, decrease inflammation, improves nerve function, regulates calcium and metabolism, improve T-cell functions, may be beneficial for some patients with erectile dysfunction and lastly, prevents the release of something called “arachidonic acid” from cells. Arachidonic acid, or AA, is what Series 2 prostaglandins, or the “bad” prostaglandins, are made from)
Fructan (Fructans are polymers of fructose and are naturally produced by 15% of flowering plant species and consist of inulin other selected vegetable fibers. They are known to strengthen the immune system support a healthy circulatory (blood) system in that they lower total cholesterol in the blood, lower low-density lipoproteins (LDL), and lower triglycerides, while raising high-density lipoproteins (HDL). Research has shown shrinkage of cancerous tumors, and blood diseases. Fructans have established their efficacy in decreasing cancers of the colon.
They do not require insulin for digestion (and therefore do not produce an energy depression before or after digestion as sucrose and glucose. Hyperglycemia inhibitors when taken to replace the natural conventional sugars due to the absence of blood-glucose spikes, and have sixty-two and one-half percent (62.5%) fewer calories than other carbohydrates such as starches, fructose, glucose, and sucrose.
Fermentation by the human colonic microflora leads to a shift in the composition of the indigenous bacterial ecosystem, in favor of health-promoting bifidobacteria. In addition to their dietary fiber effects on improved bowel regularity, several physiological advantages are linked to their specific pattern of fermentation in the colon, including improved mineral absorption, enhanced natural host defenses and colonic protection, improved gut health, and beneficial influence on appetite regulation.
Black garlic extract on cellular immune function in mice
Garlic is a plant containing a variety of organic sulfur compounds, one of the most important active ingredient is allicin. With garlic to treat infections, gunshot wounds, snake bites, diarrhea, rheumatism, heart disease, diabetes, and other physiological dysfunction in the experiment also found that with lowering blood pressure, lowering blood pressure, lowering blood sugar, anti-tumor, anti-bacterial , antioxidant and immunomodulatory effects. Organic sulfur compounds of garlic sources of some human tumors such as prostate cancer, breast cancer, colon cancer, lung cancer, bladder cancer, and so have a good inhibition. The chemical composition of garlic is very complex, biologically active ingredients of over one hundred kinds. One of the most important and unique is the very high content of organic sulfur compounds, and other high-sulfur-containing vegetables such as onions, broccoli four times. In recent years, Japanese researchers found that control humidity and temperature, normal white garlic into black garlic can be treated. Black garlic in addition to the odor of garlic, with a sweet, good taste, no longer treated directly for human consumption. Of studies have reported that black garlic white garlic contains more amino acids, organic sulfur and S-allyl-L-cysteine, and white garlic compared to more application prospects. However, there is no report about black garlic on immune function at home and abroad. To this end, the application of the experimental self-made black garlic extract solution by intraperitoneal injection to mice, to observe the role of the black garlic extract as an immunomodulatory agent, to provide further theoretical and experimental basis for the practical application of the black garlic.
Experimental materials
One, the main reagent
Black garlic extract (powder, Professor Sasaki from Hirosaki University in Japan and even as gifts), RPMI 1640 medium, fetal calf serum, 0.01 M PBS buffer, red blood cell lysates of LDH substrate solution, the Griess reagent, IL-2, FN -gamma, IL-4 and TNF-alpha ELISA kit. Second, the experimental animals
BALB / c mice, 6-8 weeks of age, SPF grade, purchased from Shanghai Institute of Experimental Animal Center.
Experimental methods
An experimental animal grouping and processing
40 BALB / c mice were randomly divided into 8 groups, 1-7 groups for the experimental group, Group 8 as the control group. Black garlic extract with saline preparation of 1mg/ml solution. The experimental group daily intraperitoneal injection of 100μl of the black garlic extract solution, the control group, intraperitoneal injection of 100μl of saline. Continuous infusion for five days. 1-7 days after the last injection, the group began a day, a group of mice were killed, eight groups of mice were sacrificed 7 days. Second, the sample collection and testing Preparation of
1, spleen cells isolated from suspension
The mice were sacrificed by cervical dislocation, the conventional preparation of spleen cell suspension, adjusting the cell concentration to 1 × 107 / ml.
2, the ELISA assay spleen cell culture supernatant of Th1 cytokines IL-2 and TNF-α and IFN-γ and Th2 cytokines IL-4 level
3 and LDH release assay of NK cell activity
4, the Griess assay spleen cell culture supernatant NO levels
5, RT-PCR semi-quantitative detection of spleen cells FasL mRNA expression levels of Third, the statistical processing
The experimental data SPSS16.0 statistics analysis software application t test was used for statistical treatment, P < 0.05, significant difference.
Result
NK cell cytotoxic activity of test results
BALB / c mice injected with black garlic extract is given in five days in a row, the experimental and control groups of mice NK cell activity, the results show that: compared with the control group (5.82 ± 1.38%), black garlic extract processing 4-6 days later, the mice NK cell activity increased significantly and reached its peak in the first 5 days (11.21 ± 1.17%), and then decreased.
2, spleen cell culture supernatant cytokine levels in the test results
In this study, we examined the Th1-type cytokines of IL-2, IFN-gamma and TNF-α and Th2 cytokines IL-4 secretion levels. We found that mice were given continuous infusion of 5 dark garlic extract, the level of IL-2 secretion in 6 days after treatment significantly increased with the control group (20.14 ± 3.88 pg / ml) compared to a significant statistically significant (P < 0.05). Of TNF-α levels from one day after treatment significantly increased (P < 0.01), peak (68.73 ± 0.64 pg / ml) in the first three days, then decreased until 7 days, were higher than control group (27.36 ± 8.30 pg / ml). Of IFN-γ secretion level after the end of the handle significantly increased, which in the first 3 days to reach peak (105.05 ± 55.73 pg / ml of), followed by drop, to the 7th day close to the control group level (17.38 ± 1.19 pg / ml of) . Th2 cytokines IL-4 secretion level after treatment has been lower than the control group (19.98 ± 2.13 pg / ml) on days 3, 4, 5 of which expression levels were significantly lower than the control group (P < 0.01) , but the change in the baseline scope.
3, spleen cell culture supernatant NO content in the test results
From the black garlic extract after 3 days to 7 days, the mice NO2-is always at a higher level compared with the control group (11.58 ± 0.86μM), there were significant differences (P < 0.001 ).
4, spleen cells from FasL mRNA expression level of the test results
By semi-quantitative RT-PCR technology to detect FasL mRNA in the level of mouse spleen cells. After processing of one day to seven days, the days spleen cells of FasL mRNA expression levels compared to control group showed no significant difference (P> 0.05).
Conclusion
1, the black garlic extract can enhance the cytotoxic activity of NK cells.
2,FasL mRNA expression levels are not increased, suggesting that black garlic extract is not induced killer cells through Fas / FasL pathway to exert its effect.
3, the black garlic extract can spleen cells of NO secretion levels were significantly increased, suggesting that black garlic extract may enhance the role of the innate immune cell activity of macrophages.
4, the black garlic extract can enhance IL-2 and IFN-γ, TNF-alpha generation level, suggesting that the black garlic extract can promote Th1-type immune response.